'Dntp' web sites
Ecological archives e087.111.a1
esapubs.org 2013-02-15
dntp , and QIAGEN Hotstart Taq. Thermal cycling included a 15 min hold at 95 C followed by 25 cycles of 94 C for 30 s, 48 C for 60 s, 68 C for 90 s, and a final extension at 72 C for 7 min. PCR products were cleaned using the QIAquick or MinElute PCR purification spin columns Qiagen, Valencia, California, USA. Cleaned PCR products were quantified using Hoechst 33258 fluorescent dye in a Turner Quantech fluorometer. Ten L sequencing
esapubs.org 2013-02-15
dntp , and QIAGEN Hotstart Taq. Thermal cycling included a 15 min hold at 95 C followed by 25 cycles of 94 C for 30 s, 48 C for 60 s, 68 C for 90 s, and a final extension at 72 C for 7 min. PCR products were cleaned using the QIAquick or MinElute PCR purification spin columns Qiagen, Valencia, California, USA. Cleaned PCR products were quantified using Hoechst 33258 fluorescent dye in a Turner Quantech fluorometer. Ten L sequencing
Iclip. transcriptome.wide mapping of protein.rna.. protocol
www.jove.com 2015-02-25
dntp mix 10mM. For each experiment or replicate, use a different Rclip primer containing individual barcode sequences see 14. Incubate for 5 min at 70 C before cooling to 25 C. Add 2.75 mu;l RT mix 2 mu;l 5x RT buffer; 0.5 mu;l 0.1M DTT; 0.25 mu;l Superscript III reverse transcriptase Invitrogen. Incubate 5 min at 25 C, 20 min at 42 C, 40 min at 50 C and 5 min at 80 C before cooling to 4 C. Add 90 mu;l TE buffer, 0.5 mu;l
www.jove.com 2015-02-25
dntp mix 10mM. For each experiment or replicate, use a different Rclip primer containing individual barcode sequences see 14. Incubate for 5 min at 70 C before cooling to 25 C. Add 2.75 mu;l RT mix 2 mu;l 5x RT buffer; 0.5 mu;l 0.1M DTT; 0.25 mu;l Superscript III reverse transcriptase Invitrogen. Incubate 5 min at 25 C, 20 min at 42 C, 40 min at 50 C and 5 min at 80 C before cooling to 4 C. Add 90 mu;l TE buffer, 0.5 mu;l
Gsdi small grants program. gsdi
www.gsdi.org 2012-04-16 ⚑news ⚑tech ⚑enterprise
dntp SDI Workshop Kenya. Foodlink Workshop on Data Sharing and Stakeholder Agreement Development Jamaica. Ministry of Lands Environment Continuation of work on 2004 grant topic Grant Update The ultimate objective is to make geospatial datasets readily available via the portal to various stakeholders inclusive of decision makers when it is required. At the touch of a button, datasets on geology, topography and socioeconomic
www.gsdi.org 2012-04-16 ⚑news ⚑tech ⚑enterprise
dntp SDI Workshop Kenya. Foodlink Workshop on Data Sharing and Stakeholder Agreement Development Jamaica. Ministry of Lands Environment Continuation of work on 2004 grant topic Grant Update The ultimate objective is to make geospatial datasets readily available via the portal to various stakeholders inclusive of decision makers when it is required. At the touch of a button, datasets on geology, topography and socioeconomic
Retrovirology. full text. rapamycin.induced inhibition of htlv.i ltr activity [..]
www.retrovirology.com 2011-05-30 ⚑tech
dntp , 0.12 956;M each oligonucleotide and 0.02 units Pfu DNA polymerase. A single incubation of 96 C for 45 s was followed by 30 cycles comprising 96 C for 45 s, 37 C for 45 s and 72 C for 10 min and an additional single extension step at 72 C for 10 min Oligonucleotides used to generate CR 916;EI.CAT were IR 5 tta gag gcc tCA GAC TTC TGT TTC TCG G3 and IF 5 gta gcg ata tcA GCA CCG GCT CGG G3 ; CR 916;EII.CAT were IIR 5 tta gag
www.retrovirology.com 2011-05-30 ⚑tech
dntp , 0.12 956;M each oligonucleotide and 0.02 units Pfu DNA polymerase. A single incubation of 96 C for 45 s was followed by 30 cycles comprising 96 C for 45 s, 37 C for 45 s and 72 C for 10 min and an additional single extension step at 72 C for 10 min Oligonucleotides used to generate CR 916;EI.CAT were IR 5 tta gag gcc tCA GAC TTC TGT TTC TCG G3 and IF 5 gta gcg ata tcA GCA CCG GCT CGG G3 ; CR 916;EII.CAT were IIR 5 tta gag
Gsdi small grants program. gsdi
www.gsdi.org 2012-04-16 ⚑news ⚑tech ⚑enterprise
dntp SDI Workshop Kenya. Foodlink Workshop on Data Sharing and Stakeholder Agreement Development Jamaica. Ministry of Lands Environment Continuation of work on 2004 grant topic Grant Update The ultimate objective is to make geospatial datasets readily available via the portal to various stakeholders inclusive of decision makers when it is required. At the touch of a button, datasets on geology, topography and socioeconomic
www.gsdi.org 2012-04-16 ⚑news ⚑tech ⚑enterprise
dntp SDI Workshop Kenya. Foodlink Workshop on Data Sharing and Stakeholder Agreement Development Jamaica. Ministry of Lands Environment Continuation of work on 2004 grant topic Grant Update The ultimate objective is to make geospatial datasets readily available via the portal to various stakeholders inclusive of decision makers when it is required. At the touch of a button, datasets on geology, topography and socioeconomic
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