Our laboratory explores simple and rapid methods for nucleic acid analyses, with goals to provide better ways to expedite research and perform clinical diagnostic tests. In the past two decades, our laboratory developed the following technologies now commonly used in research and clinical applications:
Rapid-cycle
PCR
Real-time PCR using SYBR Green I
Fluorescent DNA melting analysis
Real-time automated detection
Dual hybridization probes
High-resolution melting analysis
Unlabeled probes and snapback primers
In order to study new methods, we often had to build prototype instruments and write our own software. Some of these have been further developed and commercialized by licensees (see examples below):
Rapid Cycler ®
LightCycler ®
HR/1
LightScanner ®
LS-32®
High-Resolution Melting Many of our current studies focus on High-Resolution
Melting Analysis. High-density data acquisition and precision temperature control are used during melting so that PCR products (amplicons) can be genotyped, scanned for mutations, or matched for sequence identity directly after PCR, just by melting! See our instrument comparisonsfigure