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Targeted Re-Sequencing
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Targeted Resequencing
Targeted resequencing allows researchers to cost-effectively sequence regions of interest without sequencing an entire genome. These captured DNA regions can then be assessed for genetic variation through bioinformatics analysis.
There are several approaches at the moment to enrich a DNA sample: PCR, long range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, the samples can be pooled using the multiplexing/bar-coding capabilities of the next generation sequencing technology.
For information on specific targeted re-sequencing technologies, follow the links below:
White Papers
- Targeted Sequencing 1: Project Design and Laboratory Techniques
- Targeted Sequencing 2: Research and Clinical Applications
- Targeted Sequencing 3: Tools of the Trade
- Targeted Sequencing 4: Data Delivery
- Targeted Sequencing 5: Exome Sequencing Cheat Sheet
Exome Sequencing
- Leveraging kits that selectively amplify only the exome, a researcher can quickly and easily identify variants occurring in the coding regions of the genome.