'Flyrm' web sites
Protocols. vassar blogs
blogs.vassar.edu 2012-03-15 ⚑blog
flyrm LAB PROTOCOL Immunocytochemistry Protocol for adult nematodes September 2010 developed by K. Susman DAY 1 1. Prepare three 6cm plates for each genotype so that they are day 5 on the first day of this procedure just past food, but not starved. Collect worms into 1.5 ml microfuge tubes by washing off plates with 1 ml M9. Spin at 6000 rpm for 30 sec. Remove most of supernatant. 2. Wash 2 times with 1 ml d H2O. Spin and remove
blogs.vassar.edu 2012-03-15 ⚑blog
flyrm LAB PROTOCOL Immunocytochemistry Protocol for adult nematodes September 2010 developed by K. Susman DAY 1 1. Prepare three 6cm plates for each genotype so that they are day 5 on the first day of this procedure just past food, but not starved. Collect worms into 1.5 ml microfuge tubes by washing off plates with 1 ml M9. Spin at 6000 rpm for 30 sec. Remove most of supernatant. 2. Wash 2 times with 1 ml d H2O. Spin and remove
Protocols. vassar blogs
blogs.vassar.edu 2012-03-15 ⚑blog
flyrm LAB PROTOCOL Immunocytochemistry Protocol for adult nematodes September 2010 developed by K. Susman DAY 1 1. Prepare three 6cm plates for each genotype so that they are day 5 on the first day of this procedure just past food, but not starved. Collect worms into 1.5 ml microfuge tubes by washing off plates with 1 ml M9. Spin at 6000 rpm for 30 sec. Remove most of supernatant. 2. Wash 2 times with 1 ml d H2O. Spin and remove
blogs.vassar.edu 2012-03-15 ⚑blog
flyrm LAB PROTOCOL Immunocytochemistry Protocol for adult nematodes September 2010 developed by K. Susman DAY 1 1. Prepare three 6cm plates for each genotype so that they are day 5 on the first day of this procedure just past food, but not starved. Collect worms into 1.5 ml microfuge tubes by washing off plates with 1 ml M9. Spin at 6000 rpm for 30 sec. Remove most of supernatant. 2. Wash 2 times with 1 ml d H2O. Spin and remove
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